control strain e coli atcc 25922  (ATCC)

Journal: Comparative Immunology Reports

Article Title: The synthetic peptide GATR-3 shows significant antibacterial and biofilm-inhibition activity against shellfish- and oyster-associated bacteria Vibrio vulnificus and Vibrio parahaemolyticus

doi: 10.1016/j.cirep.2025.200266

Figure Lengend Snippet: EC 50 results for Vibrio strains against GATR-3, LL-37-NH 2 , and Mastoparan-AF-NH 2 peptides. Vibrio vulnificus MO6 EC 50 results against A. GATR-3, B. LL-37-NH 2 , and C. Mastoparan-AF-NH 2 peptides. Vibrio vulnificus JY1701 EC 50 results against D. GATR-3, E. LL-37-NH 2 , and F. Mastoparan-AF-NH 2 peptides. Vibrio parahaemolyticus NY477 EC 50 results against G. GATR-3, H. LL-37-NH 2 , and I. Mastoparan-AF-NH 2 peptides. Vibrio parahaemolyticus SAK11 EC 50 results against J. GATR-3, K. LL-37-NH 2 , and L. Mastoparan-AF-NH 2 peptides. Escherichia coli EC 50 results against M. GATR-3, N. LL-37-NH 2 , and O. Mastoparan-AF-NH 2 peptides.

Article Snippet: Against the control strain E. coli ATCC 25922, GATR-3 was extremely potent, with an EC50 of 4.44 × 10−5 μM (1.27 × 10−4 μg/mL), confirming its broad-spectrum and potent efficacy.

Techniques:

Journal: Comparative Immunology Reports

Article Title: The synthetic peptide GATR-3 shows significant antibacterial and biofilm-inhibition activity against shellfish- and oyster-associated bacteria Vibrio vulnificus and Vibrio parahaemolyticus

doi: 10.1016/j.cirep.2025.200266

Figure Lengend Snippet: Biofilm Formation of Vibrio isolates and E. coli . Quantitative comparison of biofilm formation among Vibrio isolates and E. coli after 24 h incubation. Biofilm biomass was determined by crystal violet staining and measurement of OD₆₀₀. V. vulnificus MO6 and V. parahaemolyticus NY477 exhibited the highest biofilm-forming capacities.

Article Snippet: Against the control strain E. coli ATCC 25922, GATR-3 was extremely potent, with an EC50 of 4.44 × 10−5 μM (1.27 × 10−4 μg/mL), confirming its broad-spectrum and potent efficacy.

Techniques: Comparison, Incubation, Staining

Journal: Bioresources and Bioprocessing

Article Title: Box-Behnken optimized copper oxide nanoparticles from Thymus vulgaris potentiate efficacy against multidrug-resistant bacterial pathogens and exhibit anticancer activity

doi: 10.1186/s40643-026-01008-5

Figure Lengend Snippet: Antimicrobial susceptibility profiles and efficacy of TE-CuONPs against MDR bacterial pathogens. a Inhibition zone diameters for four MDR bacterial strains against conventional antibiotics b - e Disk diffusion assays for E. faecalis , S. aureus , E. coli , and P. aeruginosa (disk positions: 1 = tetracycline, 2 = ampicillin, 3 = norfloxacin, 4 = gentamicin), confirming MDR phenotypes with gentamicin susceptibility only. f Comparative analysis showing TE-CuONPs activity (17.5–18.5 mm) significantly exceeds TE alone (5.5–8.5 mm, p < 0.05) and approaches gentamicin efficacy (20.0–25.0 mm). g MIC and MBC values demonstrating bactericidal activity of TE-CuONPs (MBC/MIC ≤ 0.58) against S. aureus , P. aeruginosa , and E. coli , with bacteriostatic response for E. faecalis (MBC/MIC > 1.05). h Schematic proposal for antimicrobial mechanisms. Different letters indicate statistical significance ( p < 0.05, ANOVA/Tukey). TE-CuONPs exhibit multimechanistic antibacterial activity through ROS generation, membrane disruption, and cellular interference

Article Snippet: Quality control strains - E. coli ATCC 25922, S. aureus ATCC 25923, and P. aeruginosa ATCC 27853—were included in each experimental run to ensure test reliability.

Techniques: Inhibition, Diffusion-based Assay, Activity Assay, Membrane, Disruption